CCR5 Mouse Monoclonal Antibody [Clone ID: T21/8]

CAT#: AM20421PU-N

CCR5 mouse monoclonal antibody, clone T21/8, Aff - Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human Tonsil stained with CCR5 Antibody at 20 ug/ml after heat-induced antigen retrieval.

• 

  Staining of normal Human peripheral blood cells with 0.25 ug of Purified Mouse IgG1, K Isotype Control (open histogram) or 0.25 ug of Purified anti-human CCR5 antibody (T21/8) (colored histogram) followed by Biotin Anti-Mouse IgG and SAv-PE. Cells in the lymphocyte gate were used for analysis.

Specifications

Product Data

• Clone Name: T21/8
• Applications: ELISA, FC, IHC, IP, WB
• Recommended Dilution: ELISA.
  Flow Cytometry: This T21/8 (T21/8) antibody has been tested by Flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µl. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
  Immunohistochemistry on Paraffin Sections: 20 µg/ml.
  Immunoprecipitation.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human CCR5
• Specificity: Recognizes C-C Chemokine Receptor 5 (CCR5).
• Formulation: Aqeous buffer containing 0.09% Sodium Azide as preservative and may contain carrier protein/stabilizer
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens C-C motif chemokine receptor 5 (gene/pseudogene) (CCR5), transcript variant A
• Database Link:
  Entrez Gene 1234 Human
• Background: Human CD195 is a member of the G protein coupled superfamily of receptors, which acts as a receptor for a number of chemokines including RANTES and also serves as a co-receptor for the entry of HIV into cells. It plays a key role in regulating the activation and migration of leukocytes. CD195 is expressed by a subset of T lymphocytes and by monocytes.
  CD195 is rapidly phosphorylated within the C-terminal domain upon ligand stimulation. Recent studies suggest that phosphorylation at Ser337 is mediated by Protein kinase C, whereas phosphorylation at Ser349 is mediated by GPCR kinases.
• Synonyms: C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CMKBR5, CHEMR13

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Endocytosis