BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: 2G5]

CAT#: AM20433PU-N

BubR1 (BUB1B) mouse monoclonal antibody, clone 2G5, Purified

Product Images

Western Blot analysis of BUB1B expression in Hela NE using Monoclonal BUB1B antibody (clone 2G5).

• 

  Western Blot analysis of BUB1B expression in transfected 293T cell line using Monoclonal BUB1B antibody (clone 2G5).

• 

  RNAi Knockdown (Antibody validated): Western blot analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Cat#H00000701-R01V) (Lane 2) or non-transfected control (Lane 1). Blot probed with AM20433PU-N BUB1B antibody (clone 2G5). GAPDH (36.1 kDa) used as specificity and loading control.

• 

  Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

• 

  Immunoperoxidase of monoclonal BUB1B antibody on Formalin-Fixed Paraffin-Embedded Human spleen at 3 ug/ml.

• 

  Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 Rabbit purified polyclonal 1/1200 and anti-BUB1B Mouse monoclonal antibody 1/50. Signals were detected by Duolink® 30 Detection Kit 613 (Red), and nuclei were counterstained with DAPI (blue). Each Red dot represents the detection of protein-protein interaction complex.

Show all 6 images

Specifications

Product Data

• Clone Name: 2G5
• Applications: ELISA, IF, IHC, PLA, RNAi, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  PLA.
  RNAi.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: BUB1B (AAH18739, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag.
• Specificity: Recognizes Mitotic Checkpoint Kinase MAD3l (BUB1B).
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)
• Database Link:
  Entrez Gene 701 Human
• Background: BUBR1 Kinase (also called Mitotic checkpoint serine/threonine-protein kinase, BUB1 beta, MAD3/BUB1-related protein kinase, Mitotic checkpoint kinase MAD3L and SSK1) is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F, CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, it can be localized to nuclear kinetochores. This protein is highly expressed in thymus followed by spleen. Defects in BUB1B are associated with tumor formation.
• Synonyms: Protein SSK1, BUBR1, MAD3L, BUB1 beta

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle