Eph receptor B4 (EPHB4) (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 5B5]

CAT#: AM20468SU-N

Eph receptor B4 (EPHB4) (Extracell. Dom.) mouse monoclonal antibody, clone 5B5, Ascites

Product Images

Western blot analysis using anti-EphB4 monoclonal antibody against extracellular domain of human EphB4 (aa16-539).

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  Colon, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Confocal Immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using anti-EphB4 monoclonal antioby (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Product Data

• Clone Name: 5B5
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200.
  Western Blot: 1/500 - 1/2000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant extracellular fragment of Human EphB4 fused with hIgGFc tag expressed in HEK293 cell line.
• Specificity: Recognizes extracellular domain of Ephrin Type B Receptor 4 (EPHB4).
• Formulation: State: Ascites
  State: Ascites fluid containing 0.03% Sodium Azide as preservative
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens EPH receptor B4 (EPHB4)
• Database Link:
  Entrez Gene 2050 Human
• Background: EPH receptor B4 (EphB4), also known as Htk, Myk1, Tyro11 is a member of the Eph receptor family, which bind the ephrin ligand family. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The EphB4 protein binds to ephrin-B2 and plays an essential role in vascular development.
• Synonyms: Ephrin type-B receptor 4, HTK, TYRO11

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance