Cytokeratin 7 (KRT7) Mouse Monoclonal Antibody [Clone ID: 5D12]

CAT#: AM20469SU-N

Cytokeratin 7 (KRT7) mouse monoclonal antibody, clone 5D12, Ascites

Product Images

Western blot analysis using Cytokeratin-7 antibody against Hela (Lane 1), MCF-7 (Lane 2), A431 (Lane 3) and A549 (Lane 4) cell lysate.

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  Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Seminal vesicle, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Tonsil, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Flow cytometric analysis of Hela cells using Monoclonal anti-Cytokeratin-7 antibody (green) and negative control (purple).

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Specifications

Product Data

• Clone Name: 5D12
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Flow Cytometry: 1/200 - 1/400.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/400.
  Western Blot: 1/500 - 1/2000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human CK7 expressed in E. Coli.
• Specificity: Recognizes Cytokeratin 7 (KRT7).
• Formulation: State: Ascites
  State: Ascites fluid containing 0.03% Sodium Azide as preservative
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens keratin 7 (KRT7)
• Database Link:
  Entrez Gene 3855 Human
• Background: CK7 (Keratin, type II cytoskeletal 7) is a protein that in humans is encoded by the KRT7 gene. CK7 is a member of the keratin family. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
• Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin

Reference Data

• Protein Families: ES Cell Differentiation/IPS