BCL2 Mouse Monoclonal Antibody [Clone ID: BL-2]

CAT#: AM20590PU-N

BCL2 mouse monoclonal antibody, clone BL-2, Aff - Purified

Product Images

Lane 1: Rat Heart tissue Lysate Lane 2: Rat spleen muscle tissue Lysate Lane 3: Rat small intestine tissue Lysate Lane 4: Rat liver tissue Lysate

Specifications

Product Data

• Clone Name: BL-2
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1 - 2 μg/ml.
  Immunocytochemistry.
  Immunohistochemistry on frozen and paraffin sections: 0.4 - 1 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to residues 41-54pf the bcl-2 protein, conjugated to thyroglobulin.
• Specificity: This antibody reacts to BCL2.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
  State: Aff - Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant alpha
• Database Link:
  Entrez Gene 596 Human
• Background: Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.
• Synonyms: BCL2, Bcl-2 alpha

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Focal adhesion, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer