Cytokeratin 7 (KRT7) Mouse Monoclonal Antibody [Clone ID: CK-7]

CAT#: AM20610PU-N

Cytokeratin 7 (KRT7) mouse monoclonal antibody, clone CK-7, Purified

Specifications

Product Data

• Clone Name: CK-7
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1 - 2 μg/ml.
  Immunohistochemistry on frozen and paraffin sections: 2 - 4 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cytoskeletal preparation of the RT4human bladder carcinoma cell line.
• Specificity: This antibody reacts to Cytokeratin 7.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens keratin 7 (KRT7)
• Database Link:
  Entrez Gene 3855 Human
• Background: KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.
• Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin

Reference Data

• Protein Families: ES Cell Differentiation/IPS