HSP27 (HSPB1) Mouse Monoclonal Antibody [Clone ID: SJ-25]

CAT#: AM20629PU-N

HSP27 (HSPB1) mouse monoclonal antibody, clone SJ-25, Purified

Specifications

Product Data

• Clone Name: SJ-25
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 0.5 - 2 μg/ml.
  Immunohistochemistry on frozen and paraffin sections: 1 - 2 μg/ml.
  Immunocytochemistry.
• Reactivities: Human, Turkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle
• Specificity: This antibody reacts to HSPB1 / HSP27.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens heat shock protein family B (small) member 1 (HSPB1)
• Database Link:
  Entrez Gene 3315 Human
• Background: The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
• Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25

Reference Data

• Protein Pathways: MAPK signaling pathway, VEGF signaling pathway