NFH (NEFH) (Phospho and non-Phospho) Mouse Monoclonal Antibody [Clone ID: NF-200]

CAT#: AM20650PU-N

NFH (NEFH) (Phospho and non-Phospho) mouse monoclonal antibody, clone NF-200, Purified

Product Images

Immunohistochemistry on Rat brain tissue sections

Specifications

Product Data

• Clone Name: NF-200
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml.
  Immunohistochemistry on frozen and paraffin sections: 1 - 2 μg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
• Specificity: This antibody reacts to Neurofilament H (200 kD).
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens neurofilament, heavy polypeptide (NEFH)
• Database Link:
  Entrez Gene 24587 RatEntrez Gene 380684 MouseEntrez Gene 4744 Human
• Background: Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 Kd (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT)
• Synonyms: NEFH,NF-H,KIAA0845, NFH, 200 kDa neurofilament protein, Neurofilament triplet H protein, (Neuronal Marker), heavy polypeptide

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)