Paxillin (PXN) (C-term) Mouse Monoclonal Antibody [Clone ID: PAX-14]

CAT#: AM20658PU-N

Paxillin (PXN) (C-term) mouse monoclonal antibody, clone PAX-14, Purified

Specifications

Product Data

• Clone Name: PAX-14
• Applications: IF, WB
• Recommended Dilution: Western Blot: 1 - 2 μg/ml.
  Immunocytochemistry.
• Reactivities: Chicken, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: C-terminal part of recombinant chicken paxillin (amino acids
  305-559)
• Specificity: This antibody reacts to Paxillin.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens paxillin (PXN), transcript variant 2
• Database Link:
  Entrez Gene 19303 MouseEntrez Gene 360820 RatEntrez Gene 5829 Human
• Background: The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.
• Synonyms: FLJ16691

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, VEGF signaling pathway