S100B Mouse Monoclonal Antibody [Clone ID: SA-12]

CAT#: AM20665PU-N

S100B mouse monoclonal antibody, clone SA-12, Purified

Product Images

Lane 1: Rat small intestine tissue lysate Lane 2: Human placenta tissue lysate

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  Lane 1: Rat small intestine tissue lysate: Lane 2: Human placenta tissue lysate:

Specifications

Product Data

• Clone Name: SA-12
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Western Blot: Use at 2 µg/ml with the appropriate system to detect S-100b in cells and tissues.
  Immunohistochemistry on Paraffin Sections: Use at 1-2 μg/ml to detect S-100b in formalin fixed and paraffin embedded tissues.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bovine brain S-100b.
• Specificity: This antibody recognizes S100B. No cross reactivity with other proteins.
• Formulation: 1.2% Sodium Acetate with 2 mg BSA and 0.01 mg Sodium Azide as preservative.
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% Sodium Acetate or neutral PBS
• Concentration: 0.1 mg/ml (after reconstitution with 1 ml PBS)
• Purification: Affinity chromatography
• Database Link:
  Entrez Gene 525716 Bovine
• Background: S100 calcium binding protein B or S100beta is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery.1 S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al. (1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.
• Synonyms: Protein S100-B, S100 beta, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, Astrocyte Marker