SMAD4 Mouse Monoclonal Antibody [Clone ID: IMD-89]

CAT#: AM20667PU-N

SMAD4 mouse monoclonal antibody, clone IMD-89, Purified

Specifications

Product Data

• Clone Name: IMD-89
• Applications: IF, WB
• Recommended Dilution: Western Blot: 2 - 4 μg/ml.
  Immunocytochemistry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human Smad4 (DPC4).
• Specificity: This antibody reacts to SMAD4.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens SMAD family member 4 (SMAD4)
• Database Link:
  Entrez Gene 4089 Human
• Background: SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes. Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Mutational inactivation of SMAD4 causes TGF-beta unresponsiveness and gave a basis for understanding the physiologic role of this gene in tumorigenesis. Mutations in DPC4 (SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases.
• Synonyms: SMAD family member 4, SMAD-4, SMAD 4, MADH4, MAD homolog 4, DPC4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway