Vinculin (VCL) Mouse Monoclonal Antibody [Clone ID: VIN-54]

CAT#: AM20685PU-N

Vinculin (VCL) mouse monoclonal antibody, clone VIN-54, Purified

Specifications

Product Data

• Clone Name: VIN-54
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1 - 2 μg/ml.
  Immunohistochemistry on frozen sections: 2 - 4 μg/ml.
• Reactivities: Chicken, Human, Mouse, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human vinculin, purified from uterus
• Specificity: This antibody reacts to Vinculin.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS.
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens vinculin (VCL), transcript variant 2
• Database Link:
  Entrez Gene 22330 MouseEntrez Gene 305679 RatEntrez Gene 7414 Human
• Background: Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane . both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95%). Vinculinis mapped to 10q22.1-q23.
• Synonyms: Metavinculin

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton