alpha Actinin (ACTN1) Mouse Monoclonal Antibody [Clone ID: SA-20]

CAT#: AM20694PU-N

alpha Actinin (ACTN1) mouse monoclonal antibody, clone SA-20, Purified

Specifications

Product Data

• Clone Name: SA-20
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Western Blot: 0.25-0.5 μg/ml.
  Immunohistochemistry on paraffin* sections: 0.5 - 1.0 μg/ml (*Boiling the sections is required).
  Immunohistochemistry on Frozen 1.0 - 2.0 μg/ml
• Reactivities: Human, Mouse, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rabbit skeletal alpha-actinin
• Specificity: This antibody reacts to Alpha-actinin-1.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens actinin alpha 1 (ACTN1), transcript variant 2
• Database Link:
  Entrez Gene 81634 RatEntrez Gene 109711 MouseEntrez Gene 87 Human
• Background: Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.
• Synonyms: Alpha actinin cytoskeletal isoform, Non-muscle alpha-actinin 1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction