beta Actin (ACTB) (Loading Control) Mouse Monoclonal Antibody [Clone ID: G043]

CAT#: AM20709PU-N

beta Actin (ACTB) (Loading Control) mouse monoclonal antibody, clone G043, Aff - Purified

Product Images

Western Blot analysis of Beta-Actin in 293 Clles. 20 ug of total cell lysates were bloted with Monoclonal Mouse anti beta-Actin antibody (G043).

Specifications

Product Data

• Clone Name: G043
• Applications: IF, WB
• Recommended Dilution: Western blot (1/1,000-1/4,000)
  Immunofluorescence (1/00).
  It is very useful as a Loading Control for quantitative western blotting.
• Reactivities: All Species
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: KLH-coupled Beta-cytoplasmic Actin N-terminal peptide.
• Specificity: Recognize 43KDa beta-Actin from Human.
• Formulation: PBS, pH 7.4 containing 0.05% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography.
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens actin beta (ACTB)
• Database Link:
  Entrez Gene 60 Human
• Background: Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
• Synonyms: Actin cytoplasmic 1, Beta-Actin

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis