c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: 19C2]

CAT#: AM20785AG-L

c-Myc (MYC) mouse monoclonal antibody, clone 19C2, Agarose

Product Images

HEK 293T cells were transfected by Myc-tagged protein, and 100 μl cell lysate (about 100 μg total protein) was incubated with 30 μl 50% slurry of Anti-Myc Agarose for 3 h at 4°C. After washing, the beads were eluted by 30 μl elution buffer twice. After neutralization of the eluant, 6 μl 6× SDS loading buffer was added. Then 20 μl sample was subjected to the SDS-PAGE. Blot was probed with Anti-Myc-Tag Mouse mAb. Lane 1: 1st Elution with elution buffer. Lane 2: 2nd Elution with elution buffer.

Specifications

Product Data

• Clone Name: 19C2
• Applications: IP
• Recommended Dilution: Immunoprecipitation.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody reacts to c-Myc Epitope Tag (EQKLISEEDK).
• Formulation: 50% slurry in storage buffer (1 PBS, pH 7.4, containing 0.09% sodium azide)
  Recommended elution buffer: 0.2 M Glycine, pH 2.5
  Label: Agarose
  State: Liquid purified Ig fraction
• Conjugation: Agarose
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens v-myc avian myelocytomatosis viral oncogene homolog (MYC)
• Database Link:
  Entrez Gene 4609 Human
• Synonyms: myc tag, myc-tag, c-myc tag

Reference Data

• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway