GFP Mouse Monoclonal Antibody [Clone ID: GFP. C2]

CAT#: AM20839PU-N

GFP mouse monoclonal antibody, clone GFP. C2, Aff - Purified

Product Images

Lane 1: Negative Control. Lane 2: 1 g GFP Fusion Protein Lysate Lane 3: 10 g GFP Fusion Protein Lysate

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  Antibody working Cont: 1/1000 from 1 mg/ ml stock Cont.

Specifications

Product Data

• Clone Name: GFP. C2
• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA.
  Western Blot (ECL): 1/1000 for 1 hour at RT.
  Dot Blot.
  Immunoprecipitation.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates.
• Specificity: Recognizes native and denatured forms of GFP and its variants EGFP, YFP,EYFP, and CFP.
  Sensitivity: 1-10 ng of purified GFP or GFP fusion proteins (Western blot developed with ECL).
• Formulation: 10mM PBS, PH 7.2 with 0.09% Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Background: The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wild type GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
• Synonyms: Yellow Fluorescent Protein