E Cadherin (CDH1) Mouse Monoclonal Antibody [Clone ID: 3F4]

CAT#: AM20867PU-N

E Cadherin (CDH1) mouse monoclonal antibody, clone 3F4, Purified

Product Images

Western Blot analysis of CDH1 expression in transfected 293T cell line by CDH1 monoclonal antibody, clone 3F4.

• 

  Immunoperoxidase of monoclonal antibody to CDH1 on formalin-fixed paraffin-embedded human stomach (antibody concentration 3 ug/ml). Formalin-Fixed, Paraffin-Embedded Human Stomach stained with CD324 / Cadherin-1 Antibody (Clone 3F4) at 3 ug/ml.

• 

  Formalin-Fixed, Paraffin-Embedded Human Lung stained with CD324 / Cadherin-1 Antibody (Clone 3F4) at 3 ug/ml after heat-induced antigen retrieval.

• 

  Formalin-Fixed, Paraffin-Embedded Human Spleen stained with CD324 / Cadherin-1 Antibody (Clone 3F4) at 3 ug/ml after heat-induced antigen retrieval.

• 

  Proximity Ligation Analysis (PLA) of protein-protein interactions between EGFR and CDH1 HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1/1200 and anti-CDH1 mouse monoclonal antibody 1/50.

Show all 5 images

Specifications

Product Data

• Clone Name: 3F4
• Applications: ELISA, IF, IHC, PLA, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 3 µg/ml.
  PLA.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein, CDH1 (NP_004351, 381 a.a. ~ 481 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to Cadherin 1, Type 1, E-cadherin (epithelial) (CDH1).
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens cadherin 1 (CDH1), transcript variant 1
• Database Link:
  Entrez Gene 999 Human
• Background: E Cadherin (CDH1) is a classical cadherin from the cadherin superfamily. It is a calcium dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
• Synonyms: Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer