IRAK (IRAK1) Mouse Monoclonal Antibody [Clone ID: 3F7]

CAT#: AM20904PU-N

IRAK (IRAK1) mouse monoclonal antibody, clone 3F7

Product Images

IRAK1 monoclonal antibody clone 3F7 Western Blot analysis of IRAK1 expression in K-562.

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  Detection limit for recombinant GST tagged IRAK1 is approximately 0.1 ng/ml as a capture antibody.

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  Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell (antibody concentration 10 ug/ml).

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Specifications

Product Data

• Clone Name: 3F7
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: IRAK1 (AAH54000, 530 a.a. ~ 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody recognizes Interleukin 1 Receptor-Associated Kinase 1 (IRAK1).
• Formulation: PBS, pH 7.2
  State: Liquid purified Ig fraction from Ascites.
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens interleukin 1 receptor associated kinase 1 (IRAK1), transcript variant 2
• Database Link:
  Entrez Gene 3654 Human
• Background: IRAK1, an IRAK type protein kinase, is a signal transducer for the immune response Toll-like receptor and interleukin-1 (IL-1) receptor signaling cascades. IRAK1 is recruited to the IL-1 receptor, phosphorylated and progressively degraded in response to IL-1 induction. The protein also has the ability to autophosphorylate. IRAK1 is immediately downstream of the MyD88 adapter protein and immediately upstream of TRAF6. IRAK1 (-/-) mice have impaired responses to interleukin-18. At least two mRNA transcript variants have been identified - 2.0 and 3.6 kb.
• Synonyms: IRAK-1, IRAK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway, Toll-like receptor signaling pathway