SHC (SHC1) Mouse Monoclonal Antibody [Clone ID: 3F4]

CAT#: AM20940PU-N

SHC (SHC1) mouse monoclonal antibody, clone 3F4

Product Images

SHC1 monoclonal antibody clone 3F4 Western Blot analysis of SHC1 expression in A-431.

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  Detection limit for recombinant GST tagged SHC1 is approximately 0.03 ng/ml as a capture antibody.

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  Human Adipocytes: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Colon, myenteric plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to SHC1 on A-431 cell (antibody concentration 10 ug/ml).

• Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1/1200 and anti-SHC1 mouse monoclonal antibody 1/50.

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Specifications

Product Data

• Clone Name: 3F4
• Applications: ELISA, IF, IHC, PLA, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 1 µg/ml.
  PLA.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: SHC1 (AAH33925, 171 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody recognizes SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1).
• Formulation: PBS, pH 7.2
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens SHC adaptor protein 1 (SHC1), transcript variant 3
• Database Link:
  Entrez Gene 6464 Human
• Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3, SHCA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Chemokine signaling pathway, Chronic myeloid leukemia, Dilated cardiomyopathy, ErbB signaling pathway, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis