ADAM17 Mouse Monoclonal Antibody [Clone ID: 1F6]

CAT#: AM20948PU-N

ADAM17 mouse monoclonal antibody, clone 1F6

Product Images

Detection limit for recombinant GST tagged ADAM17 is approximately 3 ng/ml as a capture antibody.

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  Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Proximity Ligation Analysis (PLA) of protein-protein interactions between TGFA and ADAM17. HeLa cells were stained with anti-TGFA rabbit purified polyclonal 1/1200 and anti-ADAM17 mouse monoclonal antibody 1/50.

Specifications

Product Data

• Clone Name: 1F6
• Applications: ELISA, IF, IHC, PLA, WB
• Recommended Dilution: ELISA.
  PLA.
  Western Blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant protein, ADAM17 (NP_003174, 215 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to A Disintegrin And Metalloproteinase Domain 17 (ADAM17/TACE).
• Formulation: PBS, pH 7.2
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ADAM metallopeptidase domain 17 (ADAM17)
• Database Link:
  Entrez Gene 6868 Human
• Background: Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-a is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-a. TNF-a converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association. ADAM17 is a Metallopeptidase M12B type protease.
• Synonyms: CSVP, TACE

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease, Epithelial cell signaling in Helicobacter pylori infection, Notch signaling pathway