Vitamin D Receptor (VDR) Mouse Monoclonal Antibody [Clone ID: 2F4]

CAT#: AM20955PU-N

Vitamin D Receptor (VDR) mouse monoclonal antibody, clone 2F4

Product Images

Detection limit for recombinant GST tagged VDR is approximately 1 ng/ml as a capture antibody.

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  Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 2F4
• Applications: ELISA, IHC
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein, VDR (AAH60832, 1 a.a. ~ 428 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to Vitamin D Receptor (VDR).
• Formulation: PBS, pH 7.2
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens vitamin D (1,25- dihydroxyvitamin D3) receptor (VDR), transcript variant 1
• Database Link:
  Entrez Gene 7421 Human
• Background: The vitamin D receptor, a NR1 Thyroid Hormone-Like Receptor, is activated by calcitriol, the active hormonal form of vitamin D. The activated receptor binds DNA at a specific vitamin D-responsive element and promotes transcription of vitamin D target genes such as osteocalcin, the most abundant noncollagenous protein in bone. The vitamin D receptor affects growth, bone formation, and female reproduction. For example, this receptor modulates the growth and differentiation of keratinocytes and mediates the beneficial effects of vitamin D on psoriatic skin. It also inhibits hormone secretion by and proliferation of parathyroid cells. The vitamin D receptor functions either as a homodimer or as a heterodimer of vitamin D and retinoid acid X receptor subunits. Inactivation of the receptor leads to hypocalcemia and Rickets-alopecia syndrome. Recently, the vitamin D receptor has been shown to function as a receptor for the secondary bile acid lithocholic acid (LCA), which is hepatotoxic and a potential enteric carcinogen. Activation of VDR by LCA or vitamin D induced expression in vivo of CYP3A, a cytochrome P450 enzyme that detoxifies LCA in the liver and intestine. An alternatively spliced vitamin D receptor that has an altered ligand-binding domain has been isolated from rats.
• Synonyms: VDR, 1,25-dihydroxyvitamin D3 receptor

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors