SIRT1 (456-555) Mouse Monoclonal Antibody [Clone ID: 7B7]

CAT#: AM20993PU-N

SIRT1 (456-555) mouse monoclonal antibody, clone 7B7

Product Images

SIRT1 monoclonal antibody, clone 7B7 Western Blot analysis of SIRT1 expression in Hela NE.

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  Detection limit for recombinant GST tagged SIRT1 is approximately 0.1 ng/ml as a capture antibody.

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  Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to SIRT1 on HeLa cell (antibody concentration 10 ug/ml).

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Specifications

Product Data

• Clone Name: 7B7
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein, SIRT1 (AAH12499, 456 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to Sirtuin-1 (SIRT1)at aa 456-555.
• Formulation: PBS, pH 7.4
  State: Liquid purfied Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Protein A Chromatography
• Gene Name: Homo sapiens sirtuin 1 (SIRT1), transcript variant 2
• Database Link:
  Entrez Gene 23411 Human
• Background: The SIRT family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes including transcriptional regulation, cell cycle progression, DNA damage repair and aging. Sirtuins utilize NAD+ as a substrate to catalyze deacetylation of specific acetylated protein and histones. SIRT1 has been shown to be induced by caloric restriction (CR) in several tissues and SIRT1 mediated deacetylase activity attenuates B-amyloid content in the brain during CR. SIRT1 also regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53.
• Synonyms: SIRT-1, SIR2-like protein 1, SIR2L1, sirtuin type 1, sirtuin 1, sirtuin-1, SIR2alpha

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors