CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: PD7/26/16 + 2B11]

CAT#: AM21059PU-N

CD45 (PTPRC) mouse monoclonal antibody, clone PD7/26/16 + 2B11, Purified

Product Images

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: PD7/26/16 + 2B11
• Applications: FC, IF, IHC
• Recommended Dilution: Flow Cytometry.
  Immunofluorescence.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: pD7/26/16: Human peripheral blood lymphocytes maintained in T cell growth factor.
  2B11: Isolated neoplastic cells from T cell lymphoma.
• Specificity: Recognizes CD45.
  Cellular Localization: Cell membrane.
• Formulation: 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Protein G Chromatography
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens protein tyrosine phosphatase, receptor type C (PTPRC), transcript variant 1
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45 (PTPRC) is a family of high-molecular weight glycoproteins uniquely expressed on the surface of all leukocytes and their hematopoietic progenitors. The CD45 family consists of multiple members that are all products of a single gene. Structurally, CD45 is composed of one fibronectin extracellular domain, one transmembrane domain, and two cytoplasmic tyrosine phosphatase domains. It is essential for B- and T-cell activation. For both cell-types, CD45 has been shown to interact with kinases including Lyn, src family kinase Lck, and Janus-family kinases. In T cells, CD45 is required for the initiation of receptor signaling by dephosphorylating a negative regulatory tyrosine in the C-terminal tail of Lck. CD45 may also negatively regulate Lck by dephosphorylating the tyrosine in the activation loop, thereby attenuating Lck activity.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200
• Note: Molecular Weight: 220, 205, 190, 180 kDa

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Phosphatase, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Fc gamma R-mediated phagocytosis, Primary immunodeficiency, T cell receptor signaling pathway