Lamin A (LMNA) Mouse Monoclonal Antibody [Clone ID: EM-11]

CAT#: AM26004PU-N

Lamin A (LMNA) mouse monoclonal antibody, clone EM-11, Aff - Purified

Product Images

Immunofluorescence staining of lamin C (red) in HeLa cells by monoclonal antibody EM-11. Actin decorated by phalloidin-Alexa Fluor?488 (green), DNA stained by DAPI (blue).

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  Immunofluorescence staining of lamin C (green) in mouse EL-6 fibroblasts by monoclonal antibody EM-11. DNA stained by DAPI (blue).

Specifications

Product Data

• Clone Name: EM-11
• Applications: IF, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Western blot.
  Immunocytochemistry (paraformaldehyde fixation possible).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recognizes lamin C, intermediate filament protein of nuclear lamina.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography; purity > 95% (by SDS-PAGE)
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens lamin A/C (LMNA), transcript variant 2
• Database Link:
  Entrez Gene 16905 MouseEntrez Gene 4000 Human
• Background: Lamin C is intermediate filament protein localized to the inner nuclear membrane. It is expressed predominantly in terminally differentiated cells and defines the shape and stability of nuclei in mammalian cells. Besides their structural roles, lamin proteins also regulate fundamental aspects of nuclear function and they cross-talk with cell signaling cascades and cell metabolism. Mutations of LMNA gene, encoding lamin A and C proteins, are often associated with pathogenesis of respective cell types, such as of heart myocytes.
• Synonyms: LMNA, LMN1, 70 kDa Lamin, NY-REN-32, NYREN32, Lamin-A/C, Lamin A, Lamin A + C, Nuclear Envelope Marker

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)