CEACAM1 Mouse Monoclonal Antibody [Clone ID: B3]

CAT#: AM26012PU-N

CEACAM1 mouse monoclonal antibody, clone B3, Purified


USD 365.00

2 Weeks*

Size
    • 100 ug

Other products for "CEACAM1"

Specifications

Product Data
Clone Name B3
Applications ELISA, FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use at 2-5 µg/ml.
Western blot: Use at 1-5 µg/ml. 
Flow Cytometry: Use at 10 µg/ml. 
Immunofluorescence/Immunohistochemistry: Use at 2-5 µg/ml. 
Immunohistochemistry on Paraffin Sections.
Immunoprecipitation: Use at 10 µg/IP. 
Affinity purification.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Recombinant Human soluble CEACAM-1-Fc lacking the N-domain (produced in HEK293 cells)
Specificity This Monoclonal CEACAM-1 antibody (B3) interacts solely with Human CEACAM-1 and shows no cross-reactivity with other CEACAMs expressed in Human or other species! 
B3 binds to the A1-B-Domain!
Does not react with Rat, Mouse, Dog and Cattle.
Formulation PBS, pH 7.4
State: Purified
State: Lyophilized purified IgG fraction of Cell Culture Supernatant
Reconstitution Method Restore in sterile water corresponding to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
Purification Protein G Chromatography
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Gene Name Homo sapiens carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1), transcript variant 1
Background Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1? also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). There are three soluble and seven transmembrane isoforms. The three soluble forms also contain the first two C2-type Ig like domains (aa’s 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57% aa identical to human CEACAM1? in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.
Synonyms BGP, BGP1, Biliary glycoprotein 1
Reference Data
Protein Families Druggable Genome, Transmembrane

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.