CEACAM1 Mouse Monoclonal Antibody [Clone ID: B3]

CAT#: AM26012PU-N

CEACAM1 mouse monoclonal antibody, clone B3, Purified

Product Images

Western blot analysis: Human CEACAM-1 lysate Detection utilysing 10 ug/ml

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  Sandwich ELISA: Solid phase was coated with 3 ug/ml anti CEA binding human CEACAM1- CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody mAb B3 (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.

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  IHC staining of human jejunum tissue with Monoclonal Antibody B3. CEACAM1 was detected in PFA-fixed paraffin-embedded sections of human jejunum tissue using B3 followed by staining with anti-mouse HRP-DAB and counterstaining with hematoxylin. The labeling showed week staining of CEACAM1 by B3.

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  Immunofluorescence with HeLa-CEACAM-1 cells. Cells were 4% PFA fixed (10min) and then incubated in 5% BSA/PBS for 1 h to block non-specific prtein-protein interactions. The cells were then incubated with 10ug B3-17 overnight at 4°C. The secondary antibody (green) was Alexa Flour®488 goat-anti-Mouse IgG (H+L).

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  Flow cytometry: 10 ug/ml of primary mAb B3; 250.000 Hela-human CEACAM1 cells

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Specifications

Product Data

• Clone Name: B3
• Applications: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use at 2-5 µg/ml.
  Western blot: Use at 1-5 µg/ml.
  Flow Cytometry: Use at 10 µg/ml.
  Immunofluorescence/Immunohistochemistry: Use at 2-5 µg/ml.
  Immunohistochemistry on Paraffin Sections.
  Immunoprecipitation: Use at 10 µg/IP.
  Affinity purification.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human soluble CEACAM-1-Fc lacking the N-domain (produced in HEK293 cells)
• Specificity: This Monoclonal CEACAM-1 antibody (B3) interacts solely with Human CEACAM-1 and shows no cross-reactivity with other CEACAMs expressed in Human or other species!
  B3 binds to the A1-B-Domain!
  Does not react with Rat, Mouse, Dog and Cattle.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Lyophilized purified IgG fraction of Cell Culture Supernatant
• Reconstitution Method: Restore in sterile water corresponding to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
• Purification: Protein G Chromatography
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1), transcript variant 1
• Database Link:
  Entrez Gene 634 Human
• Background: Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1? also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). There are three soluble and seven transmembrane isoforms. The three soluble forms also contain the first two C2-type Ig like domains (aa’s 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57% aa identical to human CEACAM1? in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.
• Synonyms: BGP, BGP1, Biliary glycoprotein 1

Reference Data

• Protein Families: Druggable Genome, Transmembrane