EPCAM Mouse Monoclonal Antibody [Clone ID: 323/A3]

CAT#: AM26013PU-N

EPCAM mouse monoclonal antibody, clone 323/A3, Aff - Purified

Specifications

Product Data

• Clone Name: 323/A3
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry.
  Immunoprecipitation: 1-4 μg/ml per 100-500 μg of protein in 1 ml lysate.
  Western blot: 1-4 μg/ml.
  Positive Control: A-431, MCF-7, SW480 cell lines.
  Immunohistochemistry on Paraffin Sections : 1-10 μg/ml.
  Pretreatment: Tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0).
  Positive tissue: Breast carcinoma.
  Immunocytochemistry: 1-10 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human breast cancer MCF-7 cells
• Specificity: This antibody recognizes CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.
  Negative Species: Rat.
• Formulation: PBS, pH 7.4
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens epithelial cell adhesion molecule (EPCAM)
• Database Link:
  Entrez Gene 4072 Human
• Background: CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.
• Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane