Cd9 Rat Monoclonal Antibody [Clone ID: EM-04]

CAT#: AM26023PU-N

Cd9 rat monoclonal antibody, clone EM-04, Purified

Product Images

Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody.

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  Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) purified, DAR-APC.

Specifications

Product Data

• Clone Name: EM-04
• Applications: FC, IF, IP, WB
• Recommended Dilution: Flow Cytometry: 4 μg/ml.
  Immunoprecipitation.
  Western blot: 1 μg/ml (non-reducing conditions).
  Immunocytochemistry.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Permeabilized murine bone marrow-derived mast cells (BMMC)
• Specificity: This antibody recognizes CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Purified by protein A (> 95% pure by SDS-PAGE)
• Gene Name: Mus musculus CD9 antigen (Cd9)
• Database Link:
  Entrez Gene 12527 Mouse
• Background: CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
• Synonyms: MIC3, TSPAN29, GIG2, p24, Tetraspanin-29, 5H9 antigen