Cd4 Rat Monoclonal Antibody [Clone ID: GK1.5]

CAT#: AM26029AC-N

Cd4 rat monoclonal antibody, clone GK1.5, APC

Product Images

Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) APC.

Specifications

Product Data

• Clone Name: GK1.5
• Applications: FC
• Recommended Dilution: Flow cytometry: 2 μg/ml.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Mouse CTL clone V4 cells
• Specificity: This antibody reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  Label: APC
  State: Liquid purified Ig fraction
  Label: Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Concentration: 0.5 mg/ml
• Conjugation: APC
• Gene Name: CD4 antigen
• Database Link:
  Entrez Gene 12504 Mouse
• Background: CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
• Synonyms: T-cell surface antigen T4/Leu-3