Cd4 Rat Monoclonal Antibody [Clone ID: GK1.5]
Specifications
Product Data | |
Clone Name | GK1.5 |
Applications | FC, FN, IF, IHC, IP |
Recommended Dilution | Flow Cytometry: 1 μg/million cells. Immunoprecipitation: 1-2 μg/100-500 μg of protein in 1 ml lysate. Immunohistochemistry on Frozen Sections. Immunocytochemistry: 1-4 μg/ml. Functional Application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4. |
Reactivities | Mouse |
Host | Rat |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | Mouse CTL clone V4 cells |
Specificity | This antibody reacts with an extracellular epitope of Mouse CD4 transmembrane glycoprotein (55 kDa). |
Formulation | Azide Free PBS, pH~7.4, 0.2 μm filter sterilized State: Azide Free State: Liquid purified IgG fraction |
Concentration | 1.0 mg/ml |
Purification | Protein A Chromatography |
Gene Name | CD4 antigen |
Database Link | |
Background | CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency). |
Synonyms | T-cell surface antigen T4/Leu-3 |
Reference Data |
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