CD34 Mouse Monoclonal Antibody [Clone ID: QBEnd-10]

CAT#: AM26038AF-N

CD34 mouse monoclonal antibody, clone QBEnd-10, Azide Free

Product Images

Cell surface staining of CD34 in human peripheral blood with anti-CD34 azide free.

Specifications

Product Data

• Clone Name: QBEnd-10
• Applications: FC, FN, IHC, IP, WB
• Recommended Dilution: Flow cytometry: 5-10 μg/ml.
  Immunoprecipitation.
  Western blot.
  Immunohistochemistry on paraffin sections: 1-2 μg/ml.
  Immunohistochemistry on frozen sections.
  Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human endothelial vesicles
• Specificity: This antibody reacts with Class II epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
• Formulation: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
  State: Azide Free
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD34 molecule (CD34), transcript variant 1
• Database Link:
  Entrez Gene 947 Human
• Background: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
• Synonyms: Hematopoietic progenitor cell marker

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Hematopoietic cell lineage