CD19 Mouse Monoclonal Antibody [Clone ID: AE1]

CAT#: AM26049PU-N

CD19 mouse monoclonal antibody, clone AE1, Aff - Purified

Product Images

Immunohistochemistry on frozen sections: Stainig of human tonsil with AM26049PU-N

Specifications

Product Data

• Clone Name: AE1
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1-3 μg/ml (1/50-1/200).
  Does not react on routinely processed paraffin sections.
  Has been described to work in FACS.
  Suggested Positive Control: Human tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This Monoclonal antibody AE1 recognizes the CD19 antigen also known as Leu-12.
  Antigen Distribution:
  Isolated cells: 5-50% of human peripheral blood mononuclear cells.
  Tissue sections: The antigen is expressed on B-cells and follicular dendritic cells.
• Formulation: Stock solution contains Phosphate buffered saline pH 7.2 (PBS), 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore by adding 0.5 ml distilled water.
• Concentration: 0.2 mg/ml IgG (after reconstitution)
• Purification: Affinity Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD19 molecule (CD19), transcript variant 1
• Database Link:
  Entrez Gene 930 Human
• Background: CD19, also known as Leu-12, is expressed on B cells and follicular dendritic cells. CD19 is a signal transduction molecule which regulates B-cell development, activation, and differentiation. The CD19 antigen appears early in B lymphocyte differentiation at approximately the same stage as the CD22 antigen. CD19 is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression.
• Synonyms: Leu-12, B-cell marker

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Hematopoietic cell lineage, Primary immunodeficiency