CD3E Mouse Monoclonal Antibody [Clone ID: CRIS-7]
Specifications
Product Data | |
Clone Name | CRIS-7 |
Applications | FC, IHC |
Recommended Dilution | Immunohistochemistry on frozen sections: 0.5mg/ml (1:400). Suggested positive control: Human tonsil. Has been described to work in FACS. Does not react in Immunohistochemistry on paraffin fixes cells (epitope is not fixation resistant). |
Reactivities | Human, Rhesus Monkey |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Stimulated human leukocytes |
Specificity | This antibody detects CD3 antigen on mature human T-cells. |
Formulation | Phosphate buffered saline pH 7.2 (PBS) Label: Biotin State: Lyophilized Ig fraction Stabilizer: 5 mg/ml bovine serum albumin (BSA) Preservative: 0.05% (v/v) Kathon CG |
Reconstitution Method | Reconstitute by adding 0.5ml distilled water. |
Concentration | 0.2mg/ml (reconstituted) |
Purification | Affinity purified |
Conjugation | Biotin |
Storage | Store lyophilized at 2-8°C. After reconstitution store the antibody undiluted at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | The CD3 antigen is a protein complex composed of four distinct chains (CD3γ, CD3δ and two CD3ε) that associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes. The TCR, ζ-chain and CD3 molecules together comprise the TCR complex. The CD3γ, CD3δ and CD3ε chains are highly related cell surface proteins of the immunoglobulin superfamily containing a single extracellular immunoglobulin domain. |
Synonyms | T3/Leu-4 |
Reference Data |
Documents
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