CD3E Mouse Monoclonal Antibody [Clone ID: CRIS-7]

CAT#: AM26052PU-N

CD3E mouse monoclonal antibody, clone CRIS-7, Aff - Purified

Product Images

Immunohistochemistry on Frozen Sections: Staining of human tonsil with CD3 Antibody Cat.-No AM26052PU-N

Specifications

Product Data

• Clone Name: CRIS-7
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: ≤ 0.1 μg/ml (1:2000).
  Does not react with formalin fixed cells (epitope is not fixation resistant).
  Antibody has been described to work in FACS.
  Suggested Positive Control: Human tonsil.
• Reactivities: Human, Rhesus Monkey
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Stimulated Human leukocytes
• Specificity: This antibody detects Human CD3.
  Antigen Distribution on Isolated cells: Peripheral T cells.
• Formulation: Stock solution contains 0.2 mg/ml IgG, PBS, pH 7.2 with 5 mg/ml BSA as stabilizer and 0.09% Sodium Azide as a preservative
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore by adding 0.5 ml distilled water
• Purification: Affinity Chromatography
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens CD3e molecule (CD3E)
• Database Link:
  Entrez Gene 699467 MonkeyEntrez Gene 916 Human
• Background: The CD3 antigen is a protein complex composed of four distinct chains (CD3γ, CD3δ and two CD3ε) that associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes. The TCR, ζ-chain and CD3 molecules together comprise the TCR complex. The CD3γ, CD3δ and CD3ε chains are highly related cell surface proteins of the immunoglobulin superfamily containing a single extracellular immunoglobulin domain.
• Synonyms: T3/Leu-4

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway