CD4 Mouse Monoclonal Antibody [Clone ID: EDU-2]

CAT#: AM26053BT-N

CD4 mouse monoclonal antibody, clone EDU-2, Biotin

Product Images

AM26053BT-N: human tonsil, frozen sections.

Specifications

Product Data

• Clone Name: EDU-2
• Applications: FC, IHC
• Recommended Dilution: Immunoghistochemistry on frozen sections: 0.2 mg/ml (1:1000).
  Suggested positive control: Human tonsil.
  Has been described to work in FACS.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Stimulated human leukocytes
• Specificity: This antibody recognizes the CD4 antigen, a 55kD glycoprotein (reduced and non-reduced).
• Formulation: Phosphate buffered saline pH 7.2 (PBS)
  Label: Biotin
  State: Lyophilized Ig fraction
  Stabilizer: 5 mg/ml bovine serum albumin (BSA)
  Preservative: 0.05% (v/v) Kathon CG
• Reconstitution Method: Reconstitute by adding 0.5ml distilled water.
• Concentration: 0.2mg/ml (reconstituted)
• Purification: Affinity purified
• Conjugation: Biotin
• Storage: Store lyophilized at -20°C.
  After reconstitution store the antibody undiluted at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It is mainly expressed by the T lymphocyte subset that recognizes antigens associated with self MHC class II molecules. CD4 is the primary receptor for HIV retroviruses. Like many cell surface markers, it is a member of the immunoglobulin superfamily.
• Synonyms: T-cell surface antigen T4/Leu-3
• Note: Antigen distribution:
  Isolated cells: the antibody stains approximately 20-60% of human peripheral blood mononuclear cells in flow cytometry.