Cd4 Mouse Monoclonal Antibody [Clone ID: Rib5/2]

CAT#: AM26060BT-N

Cd4 mouse monoclonal antibody, clone Rib5/2, Biotin

Product Images

Immunohistochemistry on frozen sections: Staining with AM26060BT-N on rat spleen

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  Courtesy of Dr. Lehmann, University of Rostock, Germany.

Specifications

Product Data

• Clone Name: Rib5/2
• Applications: Assay, FC, IHC
• Recommended Dilution: Immunohistochemistry on frozen sections: 0.5 μg/ml (1:800).
  Does not react on routinely processed paraffin sections.
  Has been described to work in FACS.
  Suggested positive control: Rat spleen.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Lymphoblasts
• Specificity: The antibody detects a 53kDa glycoprotein located on the cell surface. It is used for the identifcation of CD4 bearing cells, mainly T-helper / inducer lymphocytes. It can also be applied for in vivo blocking of CD4 mediated immunological reactions.
  Antigen Distribution
  Isolated Cells: T-lymphocytes and thymocytes, monocytes, macrophages.
  Tissue Sections: Rib 5/2 stains T-cell areas in the spleen and lymph nodes. In the thymus Rib 5/2 detects cortical and medullary thymocytes. It recognises tissue macrophages and Th-cells according to the CD4 distribution.
• Formulation: Stock solution contains 0.4 mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5 mg/ml BSA as a stabilizer and 0.01% Kathon as a preservative.
  Label: Biotin
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore by adding 0.5 ml distilled water.
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Rattus norvegicus Cd4 molecule (Cd4)
• Database Link:
  Entrez Gene 24932 Rat
• Synonyms: T-cell surface antigen T4/Leu-3