CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: c127]

CAT#: AM26063PU-N

CD16 (FCGR3A) mouse monoclonal antibody, clone c127, Aff - Purified

Product Images

Human placenta, staining with AM26063PU-N in Immunohistochemistry on frozen sections.

Specifications

Product Data

• Clone Name: c127
• Applications: FC, IHC, IP
• Recommended Dilution: Immunohistochemistry on Frozen Sections (Reacts mainly with granulocytes): 0.5 µg/ml (1/800).
  Has been described to work in FACS and Immunoprecipitation.
  Suggested Positive Control: Human placenta.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The antibody recognizes CD16, the low affinity receptor for IgG (FcγRIII). It detects both isoforms. The antigen is strongly expressed on granulocytes in most tissues.
  Antigen Distribution:
  Isolated cells: On flow cytometry it stains approximately 2-15% of peripheral blood lymphocytes.
  Tissue Sections: The antigen is strongly expressed on granulocytes in most tissues.
• Formulation: Stock solution contains 0.4 mg/ml IgG, Phosphate Buffered Saline pH 7.2 (PBS)
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 5 mg/ml BSA
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore by adding 0.5 ml distilled water.
• Purification: Affinity Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens Fc fragment of IgG receptor IIIa (FCGR3A), transcript variant 1
• Database Link:
  Entrez Gene 2214 Human
• Background: CD16 is expressed on the majority of NK cells, on granulocytes, monocytes and macrophages, and on a subset of T cells. CD16 is involved in antibody-dependent cellular cytotoxicity (ADCC). This molecule is encoded by FcγRIII-A or FcγRIII-B genes. The genetic heterogeneity of FcγRIII generates alternative membrane-anchored proteins with distinct signalling capacities when cross-linked by immune complexes.
  CD16 exists in two distinct forms, as a single-pass type I integral membrane protein (CD16a), and as a glycosyl phosphatidyl inositol (GPI) form (CD16b). In humans, the transmembrane form is expressed on NK cells and macrophages while the GPI linked form is expressed on granulocytes.
• Synonyms: FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, Systemic lupus erythematosus