STRO-1 Mouse Monoclonal Antibody [Clone ID: STRO-1]

CAT#: AM26067AC-N

STRO-1 mouse monoclonal antibody, clone STRO-1, APC

Product Images

Surface staining of human peripheral blood with anti-STRO-1 (STRO-1) APC.

Specifications

Product Data

• Clone Name: STRO-1
• Applications: FC
• Recommended Dilution: Flow Cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Human CD34 positive bone marrow cells.
• Specificity: This antibody detects the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
• Formulation: Tris buffered saline (TBS)
  Label: APC
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.
• Concentration: 0.1 mg/ml
• Purification: Size Exclusion Chromatography
• Conjugation: APC
• Background: STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.