Integrin alpha E (ITGAE) Mouse Monoclonal Antibody [Clone ID: Ber-ACT8]

CAT#: AM26142PU-N

Integrin alpha E (ITGAE) mouse monoclonal antibody, clone Ber-ACT8, Aff - Purified

Specifications

Product Data

• Clone Name: Ber-ACT8
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow cytometry.
  Immunoprecipitation.
  Western blot.
  Immunohistochemistry (frozen sections).
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HTLV-1 induced human T cell line MAPS16
• Specificity: This antibody recognizes CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 to -70 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) (ITGAE)
• Database Link:
  Entrez Gene 3682 Human
• Background: CD103 / Integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.
• Synonyms: Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Regulation of actin cytoskeleton