MD2 (LY96) Mouse Monoclonal Antibody [Clone ID: 4H1]

CAT#: AM26186FC-N

MD2 (LY96) mouse monoclonal antibody, clone 4H1, FITC

Specifications

Product Data

• Clone Name: 4H1
• Applications: ELISA, FN, IF, WB
• Recommended Dilution: Functional assays: Inhibits LPS binding to MD-2.
  Western blot.
  Immunoflourescence.
  Typical starting working dilution is 1:50.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Baculovirally expressed His-tagged human MD-2
• Specificity: This antibody reacts with both the monomeric and the polymeric form of sMD-2.
• Formulation: PBS
  Label: FITC
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 1% bovine serum albumin
• Concentration: 0.1 mg/ml
• Purification: Protein G
• Conjugation: FITC
• Gene Name: lymphocyte antigen 96
• Database Link:
  Entrez Gene 23643 Human
• Background:

  TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4.
  MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomers and multimers. Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells.

• Synonyms: Lymphocyte antigen 96, ESOP-1, LY96, ESOP1, MD2