RP105 (CD180) Mouse Monoclonal Antibody [Clone ID: MHR73]

CAT#: AM26255FC-N

RP105 (CD180) mouse monoclonal antibody, clone MHR73, FITC

Specifications

Product Data

• Clone Name: MHR73
• Applications: FN, IHC, IP
• Recommended Dilution: Flow cytometry: The typical starting working dilution is 1:50.
  Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
  Immunoprecipitation.
  Functional assays.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The monoclonal antibody MHR73 reacts with human RP105 (CD180)
• Formulation: PBS
  Label: FITC
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Conjugation: FITC
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: CD180 molecule
• Database Link:
  Entrez Gene 4064 Human
• Background: RP105 is a type 1 transmembrane protein of 105 kDa with extracellular leucine-rich repeats (LLRs) and a short cytoplasmic tail. RP105, assigned as CD180, is similar to Drosophila Toll in the extracellular LLRs. RP105 expression is largely restricted to immune cells including CD19+ B cells and macrophages. Histological studies showed that RP105 is expressed mainly on mature B cells in mantle zones. Antibody-mediated (MHR73) cross-linking of RP105 induces resistance against irradiation-induced apoptosis, B-cell proliferation, and up-regulation of a costimulatory molecule CD80, revealing RP105 as a potent regulator of B-cell activation. In this context it is important to note that RP105 is associated with MD1, and that it mediates B-cell surface expression and LPS recognition and signalling in cooperation with TLR4. MD-1 seems to be requisite for efficient expression of RP105.
• Synonyms: LY64