TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: 52B83]

CAT#: AM26298PU-N

TNF alpha (TNF) mouse monoclonal antibody, clone 52B83, Purified

Specifications

Product Data

• Clone Name: 52B83
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections (4): 6-µm tissue sections were fixed in acetone for 10 minutes (Ref. 4).
  Immunohistochemistry on paraffin sections (5): Tissue sections were fixed in 5% formalin embedded in paraffin and cut into 2µm sections (Ref. 5).
  Flow cytometry (3): Antibody 52B83 was used to stain soluble TNF bound to TNF receptors. Cells were fixed in PBA containing 0.2% formaldehyde (Ref. 3).
  Western blot: A reduced sample treatment and 15% SDS-Page was used. The band size observed is 17 kDa (Ref.4).
  The typical starting working dilution is 1:50.
• Reactivities: Guinea Pig, Human, Mouse, Rhesus Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity:
  The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha).
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein G
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 7124 Human
• Background:

  TNF-alpha is a homotrimeric 17 kDa protein, that interacts with either one of the two types of TNF-receptors, termed I and II, leading to receptor cross-linking and signal transduction. The receptors differ strongly in their intra-cellular signaling pathways.
  TNF-alpha was originally described as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-alpha is found to be a central mediator in many inflammatory and immunological processes. It can be induced by various products of micro-organisms and by various cytokines leading to expression of a wide variety of cytokines. The pro-inflammatory properties of TNF-alpha play a central role in several auto-immune diseases such as rheumatoid arthritis and inhibition by neutralizing molecules have been shown to be beneficial in patients.

• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin