Siglec-H Rat Monoclonal Antibody [Clone ID: 440c]

CAT#: AM26317PU-N

Siglec-H rat monoclonal antibody, clone 440c, Purified


USD 465.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name 440c
Applications FN, IHC
Recommended Dilution Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
Flow cytometry: The typical starting working dilution is 1:50.
Functional assays (inhibition).
Reactivities Mouse
Host Rat
Isotype IgG2b
Clonality Monoclonal
Specificity The monoclonal antibody 440c reacts with Siglec-H, a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). It exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.
Formulation PBS
State: Purified
State: Liquid 0.2 µm filtered Ig fraction
Stabilizer: 0.1% bovine serum albumin
Concentration 0.1 mg/ml
Purification Protein G
Background Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.