Lbp Rat Monoclonal Antibody [Clone ID: RR433-8]

CAT#: AM26336PU-N

Lbp rat monoclonal antibody, clone RR433-8, Purified

Specifications

Product Data

• Clone Name: RR433-8
• Applications: ELISA, FN, WB
• Recommended Dilution: Flow cytometry.
  Immunoassays.
  Western blot: The typical starting working dilution is 1:10.
  Functional assays: In vitro and in vivo aimed at blocking LBP.
  Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity:
  The monoclonal antibody RR433-8 reacts highly specific with mouse natural and recombinant LBP. The antibody is a type II antibody blocking the LPS binding to CD14.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: 0.1 mg/ml
• Purification: Protein G
• Gene Name: lipopolysaccharide binding protein
• Database Link:
  Entrez Gene 16803 Mouse
• Background: LPS binding protein (LBP) is an approximately 60 kDa acute phase protein that is produced by hepatocytes. This protein strongly binds to LPS and has been shown to play an important role in the handling of LPS by the host. A number of functions of LBP have been reported. First, LBP transfers LPS to the LPS receptor CD14 on mononuclear phagocytes, leading to an 100-1,000-fold increased sensitivity of the cells to LPS. Furthermore, LBP can enhance the response of CD14 negative cells by acceleration of LPS binding to soluble CD14, a complex that stimulates these cells. Next, LBP transfers LPS into High Density Lipoprotein (HDL), which effectively neutralizes its biological potency. LBP was demonstrated to protect mice from septic shock caused by LPS or gram negative bacteria.
• Synonyms: Lipopolysaccharide-binding protein