GST-Tag Mouse Monoclonal Antibody [Clone ID: GST62]

CAT#: AM26390PU-L

GST-Tag mouse monoclonal antibody, clone GST62, Purified

Product Images

Recombinant GST protein (20 ng, Lane 1) and 293 cellular lysates transfected with a GST expression vector (Lane 2) were probed with anti-GST (1 g/ml). Arrow indicates GST (~26kDa) and GST-fusion protein (~29kDa).

Specifications

Product Data

• Clone Name: GST62
• Applications: IP, WB
• Recommended Dilution: Western blot: 1/1000.
  Immunoprecipitation: 1/200.
  Immunostaining: 1/200.
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: A synthetic peptide from GST coupled to KLH
• Specificity: This antibody binds to GST-tagged recombinant protein.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Lyophilized purified IgG fraction
  Preservative: 0.05% Sodium azide
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. Expression vectors with Glutathione S-transferase (GST) tag have beed developed widely for both prokaryotic and eukaryotic systems, because GST can provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein encoded by inserted cDNA sequences. Antibodies of anti-GST tag are useful for checking protein expression using immunoblotting, immunoprecipitation and immunostaining techniques, as well as for immunoaffinity purification of protein expressed in vitro.
• Synonyms: GST26-Tag, Glutathione S-transferase Tag