CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: UB2]

CAT#: AM26668RP-N

CD95 (FAS) mouse monoclonal antibody, clone UB2, PE

Product Images

Flow cytometric analysis of human Fas expression on transfectant. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26668RP-N to the cells.

Specifications

Product Data

• Clone Name: UB2
• Applications: FC
• Recommended Dilution: Flow cytomstry: 20 μl (ready for use).
  For details see protocol below.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human Fas
• Specificity: This antibody recognizes human Fas antigen.
• Formulation: PBS
  Label: PE
  State: Liquid Ig fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% NaN₃
• Purification: Affinity chromatography on protein A
• Conjugation: PE
• Storage: Store at 2-8 °C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 355 Human
• Background: It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas antigen is a cell-surface protein that mediates apoptosis. It is expressed in various tissues including the thymus and has structural homology to a number of cell-surface receptors, including tumor necrosis factor receptor and nerve growth factor receptor.
• Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
• Note:

  This product was originally produced by MBL International.

  
  
  Protocol: Flow cytometric analysis for floating cells
  We usually use Fisher tubes or equivalents as reaction tubes for all steps described below.
  1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN 3].
  2) Resuspend the cells with washing buffer (5x10 6 cells/mL).
  3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration.
  4) Add 10 μ L of normal goat serum containing 1 mg/mL normal human IgG and 0.1% NaN 3 to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature.
  5) Add 20 μ L of PE labeled anti-Fas monoclonal antibody (UB2). Mix well and incubate for 30 minutes at room temperature.
  6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
  7) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer.
  (Positive control for Flow cytometry; transfectant)