HLA DM (HLA-DMA) Rat Monoclonal Antibody [Clone ID: M5/114]

CAT#: AM26699AF-N

HLA DM (HLA-DMA) rat monoclonal antibody, clone M5/114, Azide Free

Product Images

Surface staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.

Specifications

Product Data

• Clone Name: M5/114
• Applications: FC, FN, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 2 μg/ml.
  Immunoprecipitation.
  Western blot.
  Immunohistochemistry on Paraffin Sections.
  Immunohistochemistry on Frozen Sections.
  Functional Application: Blocking of T cell proliferative responses.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Activated C57BL/6 mouse spleen cells
• Specificity:

  The antibody reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

• Formulation: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
  State: Azide Free
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Protein-G Affinity Chromatography
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Background: MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
• Synonyms: DMA, RING6, MHC class II antigen DMA