HLA DM (HLA-DMA) Rat Monoclonal Antibody [Clone ID: M5/114]

CAT#: AM26699FC-N

HLA DM (HLA-DMA) rat monoclonal antibody, clone M5/114, FITC

Specifications

Product Data

• Clone Name: M5/114
• Applications: FC
• Recommended Dilution: Flow Cytometry: 4 µg/ml (starting point).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Activated C57BL/6 mouse spleen cells
• Specificity: The antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
• Formulation: Phosphate buffered saline (PBS), approx. pH 7.4
  Label: FITC
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: lot specific
• Conjugation: FITC
• Storage:

  Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.

• Stability: Shelf life: one year from despatch.

• Background: MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
• Synonyms: DMA, RING6, MHC class II antigen DMA