CD82 Mouse Monoclonal Antibody [Clone ID: C33]

CAT#: AM26701PU-N

CD82 mouse monoclonal antibody, clone C33, Aff - Purified

Product Images

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).

Specifications

Product Data

• Clone Name: C33
• Applications: FC, FN, IF, IHC, IP, WB
• Recommended Dilution:

  Flow Cytometry.
  Immunoprecipitation
  Western blot.
  Immunohistochemistry on Paraffin Sections.
  Immunocytochemistry.
  Functional Application: In Human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with Human T-cell leukemia virus type 1 (HTLV-1)-positive Human T-cell lines.

• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C91/PL (Human HTLV-1+ T cell line)
• Specificity: The antibody reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
• Formulation: Phosphate buffered saline (PBS), approx. pH 7.4
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Protein-A Affinity Chromatography
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 3732 Human
• Background: CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.
• Synonyms: KAI1, SAR2, ST6, TSPAN27, C33 antigen, IA4, Kangai-1, Tetraspanin-27