Piccolo (PCLO) Mouse Monoclonal Antibody [Clone ID: PCLO-01]

CAT#: AM26707RP-N

Piccolo (PCLO) mouse monoclonal antibody, clone PCLO-01, PE

Product Images

Western blot of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.

Specifications

Product Data

• Clone Name: PCLO-01
• Applications: FC
• Recommended Dilution: Flow cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant PCLO protein
• Specificity: This antibody PCLO-01 recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytomatrix of the neurons.
• Formulation: Phosphate buffered saline (PBS)
  Label: PE
  State: Liquid Ig fraction
  Stabilizer: 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA)
  Preservative: 15 mM sodium azide
• Concentration: 0.1 mg/ml
• Purification: Size-exclusion chromatography
• Conjugation: PE
• Database Link:
  Entrez Gene 27445 Human
• Background: PCLO (piccolo, also known as aczonin) is a large (more than 400 kDa) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B). In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels. It is involved in neurotransmitter release and insulin secretion.
• Synonyms: Protein piccolo, Aczonin, ACZ, KIAA0559