ARHGEF4 (143-271) Mouse Monoclonal Antibody [Clone ID: ARHGEF-08]

CAT#: AM26710RP-N

ARHGEF4 (143-271) mouse monoclonal antibody, clone ARHGEF-08, PE

Product Images

Western blot analysis of ARHGEF4 in HEK293 cells (A) and HEK293-ARHGEF4 transfectants (B) using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08).

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  Intracellular staining of ARHGEF4 in transfected HEK-293 cells using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08) PE.

Specifications

Product Data

• Clone Name: ARHGEF-08
• Applications: FC, WB
• Recommended Dilution: Flow Cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant fragment of human ARHGEF4 (amino acids 143-271)
• Specificity: This antobdy recognizes Human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42.
• Formulation: PBS
  Label: PE
  State: Liquid purified Ig fraction
  Stabilizer: 0.2% (w/v) high-grade protease free BSA
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions.
• Concentration: 0.1 mg/ml
• Purification: Size Exclusion Chromatography
• Conjugation: PE
• Database Link:
  Entrez Gene 50649 Human
• Background: ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli – stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
• Synonyms: KIAA1112, ASEF